Validação De Método Analítico Para Análise De Amitraz Em Mel Utilizando Gc/tsd

This paper describes the validation of method for analysis of the acaricide amitraz in honey using nhexane/acetone extraction, cleanup in cartridge of solid phase extraction (SPE) and analysis by gas chromatography with nitrogen-phosphorus detector (GC -TSD). The method can be used to monitor the presence of the pesticide amitraz in honey because of its good analytical parameters, precision (CV <11%) and accuracy (> -1 80%) in the range of 100-400 ng g . The limit set in the European Union, by Committee for Veterinary -1 Medicinal Products, for amitraz in honey (200 ng g ) is within this range. The response of the analytical system -1 -1 was linear between 100 and 800 ng mL with correlation coefficient of 0.9996, limit of detection (LD -7 ng g ) -1 -1 -1 and quantification (LQ -100 ng g ). The sample was fortified at three concentrations 100 ng g , 200 ng g and -1 400 ng g , giving an average recovery of 80, 88 and 92% respectively. The validated method was used to assess the quality of honey sold in the city of Araraquara/SP, in which there were no residues of amitraz.